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ORIGINAL ARTICLE
Year : 2017  |  Volume : 1  |  Issue : 2  |  Page : 63-68

Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis


Human Assisted Reproduction Center, Shanxi Women and Children's Hospital, Taiyuan 030013, China

Correspondence Address:
Xue-Qing Wu
Human Assisted Reproduction Center, Shanxi Women and Children's Hospital, Taiyuan 030013
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2096-2924.216861

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Background: This study evaluated the expression of cellular FLICE-like inhibitory protein (cFLIP) in granulosa cells (GCs) obtained from in vitro fertilization-embryo transfer patients with advanced endometriosis. Methods: A total of 267 patients with advanced endometriosis were enrolled in this study. They were divided into clinical pregnancy group (n = 114) and nonpregnancy group (n = 153). The expressions of cFLIP in mRNA and protein level were measured by real-time polymerase chain reaction (RT-PCR) and Western blotting. The related factors on the clinical pregnancy were analyzed using logistic regression analysis. Coefficients of correlation were calculated using the nonparametric rho-Spearman test. Results: The number of oocytes retrieved, fertilization rate, and cleavage rate were significantly and independently related with clinical pregnancy (P < 0.05). RT-PCR and Western blotting analysis showed that the expressions of cFLP in mRNA and protein level were significantly higher in the clinical pregnancy group than in nonpregnancy group (P < 0.05). cFLIP had a significantly positive correlation with the number of oocytes retrieved (P < 0.05) and no correlation with fertilization rate and cleavage rate (P > 0.05). Conclusion: Higher expression of cFLIP increased the pregnancy rate in women with advanced endometriosis.


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