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ORIGINAL ARTICLE
Year : 2017  |  Volume : 1  |  Issue : 4  |  Page : 233-238

Effects of cryopreservation on human sperm glycocalyx


1 Department of Applied Biology, State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East University of Science and Technology, Shanghai 200237, China
2 Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China
3 Department of Allergy, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China
4 Department of Reproductive Pharmacology, NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
5 Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 201100, China
6 Department of Andrology, Urologic Medical Center, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
7 Department of Applied Biology, State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East University of Science and Technology; Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Correspondence Address:
Yong-Lian Zhang
Shanghai Institute of Biochemistry and Cell Biology, No. 320 Yueyang Road, Shanghai 200031
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2096-2924.224914

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Background: To study the effects of cryopreservation on human sperm glycocalyx. Methods: The lectin binding profilings of sperm after freeze-thaw were compared by lectin microarray. Results: CryoSperm™ and direct fumigation were confirmed to be the optimized cryoprotectant and method by comparing the sperm recovery rate. In 91 lectins, 33 lectins were significantly changed after sperm cryopreservation. Among them, 9 lectins greatly decreased and 24 lectins mainly increased. The binding signals of MAA, PSA, ABA, and AIA were verified by FACS, and the results were consistent with that of lectin microarray. Conclusions: Sperm glycocalyx had significant changes after cryopreservation. The sialic acid, playing an important role in protecting sperm, was greatly lost, which exposed the inner carbohydrates. Thus, the glycocalyx damage due to the cryopreservation might be one of the reasons for low sperm fertility.


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