• Users Online: 635
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2019  |  Volume : 3  |  Issue : 1  |  Page : 5-10

Deficiency of antisense lncRNA Gm48853 resulted in embryonic lethality and impaired placental development in mice


1 The Institute of Reproduction and Developmental Biology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200438, China
2 Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200438, China
3 The Institute of Reproduction and Developmental Biology, Obstetrics and Gynecology Hospital; The State Key Laboratory of Genetic Engineering, MOE Key Laboratory of Contemporary Anthropology, School of Life Science, Fudan University, Shanghai 200438, China
4 The Institute of Reproduction and Developmental Biology, Obstetrics and Gynecology Hospital; Institute of Developmental Biology and Molecular Medicine; Children's Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai 200438, China

Correspondence Address:
Hong-Yan Wang
The State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, 2005 Songhu Road, Shanghai 200438
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2096-2924.255984

Rights and Permissions

Objective: Long noncoding RNAs (lncRNAs) play important roles in embryonic development and in various diseases. Gm48853 is a predicted natural antisense lncRNA gene of unknown function. The present study aimed to investigate its expression and function. Methods: Quantitative reverse transcription-polymerase chain reaction and sequencing were used to detect the expression of Gm48853 antisense lncRNA. Hematoxylin and eosin staining and pathological analysis were applied to evaluate the effects of Gm48853 mutations on embryonic/placental development. 5-Ethynyl-2'-deoxyuridine (EdU) and terminal deoxynucleotidyl transferase deoxy-UTP-nick-end labeling assays were used to analyze the status of cell proliferation and apoptosis in Gm48853- deficient embryos and placentas. Results: PiggyBac (PB) transposon insertion into the intron of Gm48853 resulted in a significant decrease in Gm48853 expression and embryonic lethality at midgestation. Homozygous Gm48853 mutations led to severe growth retardation of embryos and impaired the morphogenesis of the placental labyrinth. In addition, cell proliferation was dramatically decreased in Gm48853PB/PB embryos and placentas, and apoptosis was radically increased in the mutant embryos but not in the mutant placentas. Conclusion: Antisense lncRNA Gm48853 may regulate embryonic/placental development by enhancing cell proliferation and/or inhibiting apoptosis.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed352    
    Printed40    
    Emailed0    
    PDF Downloaded108    
    Comments [Add]    

Recommend this journal