Figure 2: Src2 and Crebbp mRNA levels in the anterior hypothalamus in young and middle-aged female rats. Rats were given two daily doses of E2 benzoate (2 μg) and were euthanized 52 h (young) or 54 h (middle aged) after the first injection of E2. Age was a significant factor, and an interaction between age and hormone levels was detected in Src2 (a). Significant effects of age, hormone level, and interactions between age and hormones were found for Crebbp (b). Significant effects of hormone within an age group are indicated by bold brackets. Significant effects of age within a hormone group are indicated by thin brackets. Data are shown as means ± standard error of the mean, n = 6 in both groups. *P < 0.01.