Figure 4: Estrogen receptor-alpha coactivator gene expression in the posterior hypothalamus in young and middle-aged female rats. Rats were given two daily doses of E2 benzoate (2 μg) and were euthanized 52 h (young) or 54 h (middle aged) after the first injection of E2. A significant effect of age was found for Src1 (a). Significant interactions between age and hormone were found for Crebbp (b). Significant effects of hormone within an age group are indicated by bold brackets. Significant effects of age within a hormone group are indicated by thin brackets. Data are shown as means ± standard error of the mean, n = 6 in both groups. *P < 0.05.