Figure 2: Efficiency of NUP107 knockdown in KGN cells and cell proliferation after transfection. (a-c) Expression of NUP107 mRNA was detected by Western Blot after transfection for 48 h (a,b), and mRNA was detected by RT-qPCR after transfection for 12 h (c). (d) Cell viability was assessed by CCK8 in the NUP107 knockdown and control groups. (e) RTCA was used to monitor changes in cell amplification after transfection for 4 days. (f-i) Cell cycle distribution was analyzed by FCM after transfection for 48 h. (j) The RT-qPCR was used to detect CDK1, CDK2, CDK4 and CDK6 expression after transfection for 12 h. FCM: Flow cytometry; RTCA: Real-time cell analysis; RT-qPCR: Reverse transcription-quantitative polymerase chain reaction; *: P < 0.05.