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  Indian J Med Microbiol
 

Figure 3: Knockdown of NUP107 has no effect on cell apoptosis or senescence state of GCs. (a) FCM was utilized to detect the apoptotic rate by PE-Annexin V/7-AAD staining after transfection for 48 h. Early apoptosis cells are stained positive for PE-Annexin V(+)/7-AAD(−), and late apoptosis cells are stained for PE-Annexin V(+)/7-AAD(+). (b) Representative micrographs (×100) of control and knockdown group cells subjected to SA-β-Gal activity assays after transfection for 48 h. Senescent cells were stained in blue. GCs: Granulosa cells; FCM: Flow cytometry; 7-AAD: 7-amino-actinomycin.

Figure 3: Knockdown of <i>NUP107</i> has no effect on cell apoptosis or senescence state of GCs. (a) FCM was utilized to detect the apoptotic rate by PE-Annexin V/7-AAD staining after transfection for 48 h. Early apoptosis cells are stained positive for PE-Annexin V(+)/7-AAD(−), and late apoptosis cells are stained for PE-Annexin V(+)/7-AAD(+). (b) Representative micrographs (×100) of control and knockdown group cells subjected to SA-β-Gal activity assays after transfection for 48 h. Senescent cells were stained in blue. GCs: Granulosa cells; FCM: Flow cytometry; 7-AAD: 7-amino-actinomycin.