Figure 4: Effects of NUP107 knockdown on receptors expression, estrogen production, and FSH sensitivity. (a) The mRNA expression of AMHR2, FSHR, LHR, ESR1 and ESR2 were detected by RT-qPCR after transfection for 12 h. (b) Expression of FSHR, AMHR2, ERa, and ERb at the protein level were tested by Western Blot. (c) Estradiol levels in cell culture media were detected by ELISA in the presence of 10 ng/mL testosterone (T) as a substrate after 48 h of transfection with and without 100 IU/mL rFSH. (d) The mRNA levels of AR, CYP17A1, CYP19A1, NR5A1, and STAR were detected by RT-qPCR. FSH: Follicle stimulating hormone; RT-qPCR: Reverse transcription-quantitative polymerase chain reaction; ELISA: Enzyme-linked immunosorbent assay.