Figure 4: Blockade of p38 signaling inhibits Gal-9-stimulated invasion of HTR8/SVneo cells. (a and b) Matrigel invasion assays of HTR8/SVneo cells treated with Gal-9 (1,000 ng/mL) in the presence or absence of the specific signaling pathway inhibitors. Images are representative of three individual experiments. (c and d) Flow cytometric analysis (d) and quantitation (c) of MMP-2 production by HTR8/SVneo cells after stimulation with or without Gal-9 (1,000 ng/mL) in the presence or absence of the specific signaling pathway inhibitors for 48 h. The flow cytometry plot is from one representative experiment. Data represent the mean ± standard error of the mean. *P ≥ 0.01, †P ≥ 0.001, compared with the control group. ‡P < 0.001, compared with the Gal-9 group.