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  Indian J Med Microbiol
 

Figure 1: The specificity of mcAb 10#B4 was determined by Western blotting (a, c, and d) and dot blot (b) assays. The loaded recombinant active defensin peptides (SPAG11E, RBD14′, and RBD15′) in (a) was 2 ng per lane and the tandem repeat antigens (RBD1, RBD14, RBD15, RBD42, MBD15, and MBD30) loaded in (b) was 1 μg per dot. The loading amount of protein extract from different tissues (caput region, segments 1–8; corpus region, segments 9–14; and cauda region, segments 15–19) was 30 μg per lane (c). (d) The recognition abilities of monoclonal antibodies 10#A5, 3#-1, 14#F6, and 10#B4 (1:1000) for recombinant full-length human SPAG11B/D and tandem-repeat antigen SPAG11E. *Tandem-repeat antigen of SPAG11E (5 ng per lane) as a positive control. Pre: E. coli cell lysate before induction as a negative control to show the background of Western blotting for the designated antibodies; Post: E. coli cell lysate after induction with IPTG to show the specific recognition of the designated antibodies to recombinant SPAG11B/D; E. coli: Escherichia coli.

Figure 1: The specificity of mcAb 10#B4 was determined by Western blotting (a, c, and d) and dot blot (b) assays. The loaded recombinant active defensin peptides (SPAG11E, RBD14′, and RBD15′) in (a) was 2 ng per lane and the tandem repeat antigens (RBD1, RBD14, RBD15, RBD42, MBD15, and MBD30) loaded in (b) was 1 μg per dot. The loading amount of protein extract from different tissues (caput region, segments 1–8; corpus region, segments 9–14; and cauda region, segments 15–19) was 30 μg per lane (c). (d) The recognition abilities of monoclonal antibodies 10#A5, 3#-1, 14#F6, and 10#B4 (1:1000) for recombinant full-length human SPAG11B/D and tandem-repeat antigen SPAG11E. *Tandem-repeat antigen of SPAG11E (5 ng per lane) as a positive control. Pre: <i>E. coli</i> cell lysate before induction as a negative control to show the background of Western blotting for the designated antibodies; Post: <i>E. coli</i> cell lysate after induction with IPTG to show the specific recognition of the designated antibodies to recombinant SPAG11B/D; <i>E. coli: Escherichia coli</i>.