Figure 2: Epitope mapping of the SPAG11E antigen protein by Western blotting. Recombinant 16-mer epitope fusion proteins were analyzed by SDS-PAGE (a) and Western blotting (b and c) assays. The cell lysate of clones S1 to S6, which produce the corresponding 16-mer epitope fusion proteins as shown in Table 1, was analyzed with antiserum (b) and monoclonal antibodies (c). All the epitope motifs in human (HumE), mouse (MouE), and rat (RatE) are presented in d, with consensus motifs highlighted by a box, and the detailed data of the epitope mapping are shown in Table 2. *Epitope motifs recognized by antiserum. C: Control; Pre: Cell lysate before induction; Po: Cell lysate after induction.