Figure 4: Localization of SPAG11E with rabbit and mouse polyclonal antibodies and mcAb 10#B4 in fluorescent immunochemical staining of the caput, corpus, and cauda sperm of rat. Nuclear staining was not done to avoid the appearance of pseudosignals. The profiles of sperm are shown in phase-contrast fields instead. Rabbit and mouse antibodies were labeled with FITC (green)-and TRITC (red)-conjugated goat IgG. The fields were observed under a ×100 objective lens. Bars, 5 μm.