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  Indian J Med Microbiol
 

Figure 5: Western blotting analysis of SPAG11E colocalized with sperm by treatments with buffers of different stringency and LL-pronase. The detail of sequential protein extract of rat sperm at caput region with buffers designated from the left to right side (left panel) was described in methods, and the protein extract was analyzed by Western blotting with mcAb 10#B4. Rat sperm of caput region was treated with LL-pronase (right panel) for 15, 30, and 45 min to remove nonnucleus materials and equal amount of pellet protein was analyzed by Western blotting analysis using mcAb 10#B4. PI: Protease inhibitor; LL-pronase: l-α-phosphatidylcholine-pronase.

Figure 5: Western blotting analysis of SPAG11E colocalized with sperm by treatments with buffers of different stringency and LL-pronase. The detail of sequential protein extract of rat sperm at caput region with buffers designated from the left to right side (left panel) was described in methods, and the protein extract was analyzed by Western blotting with mcAb 10#B4. Rat sperm of caput region was treated with LL-pronase (right panel) for 15, 30, and 45 min to remove nonnucleus materials and equal amount of pellet protein was analyzed by Western blotting analysis using mcAb 10#B4. PI: Protease inhibitor; LL-pronase: l-α-phosphatidylcholine-pronase.