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  Indian J Med Microbiol
 

Figure 4: Determination of the optimal number of reference genes for normalization. The pair-wise variations (V = Vn/n + 1) were calculated using geNorm. Pair-wise variation values < 0.15 indicate that the inclusion of an additional reference gene is not expected to substantially influence normalization.

Figure 4: Determination of the optimal number of reference genes for normalization. The pair-wise variations (V = V<sub>n</sub>/<sub>n + 1</sub>) were calculated using geNorm. Pair-wise variation values < 0.15 indicate that the inclusion of an additional reference gene is not expected to substantially influence normalization.